Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Alterations in PD-L1 Expression Associated with Acquisition of Resistance to ALK Inhibitors in ALK -Rearranged Lung Cancer

doi: 10.4143/crt.2018.486

Figure Lengend Snippet: Programmed cell death–ligand 1 (PD-L1) protein levels in the parental H3122 and its subclonal cell lines with single- and double-resistant cell lines by Western blot (band intensities were measured by densitometry using Image J and normalized to those of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) serving as a loading control) (A) and fluorescence-activated cell sorting analysis. Histograms depict PD-L1 expression (B). PD-L1 mRNA levels. The error bars indicate the standard deviation (C).

Article Snippet: Cells were resuspended in 100 μL FACS buffer and then stained with mouse anti-human PD-L1 (MIH1, BD Pharmingen, San Jose, CA) or a mouse IgG1 isotype control for 30 minutes on ice in staining buffer (2% bovine serum albumin and 0.01% sodium azide).

Techniques: Western Blot, Fluorescence, FACS, Expressing, Standard Deviation

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Alterations in PD-L1 Expression Associated with Acquisition of Resistance to ALK Inhibitors in ALK -Rearranged Lung Cancer

doi: 10.4143/crt.2018.486

Figure Lengend Snippet: Representative images of programmed cell death–ligand 1 (PD-L1) immunohistochemistry in non-small cell lung cancer patients (×400). PD-L1 score was graded as absent (score 0) (A), weak (score 1) (B), moderate (score 2) (C), or strong (score 3) (D). A score of 2 or 3 was deemed positive for PD-L1 expression.

Article Snippet: Cells were resuspended in 100 μL FACS buffer and then stained with mouse anti-human PD-L1 (MIH1, BD Pharmingen, San Jose, CA) or a mouse IgG1 isotype control for 30 minutes on ice in staining buffer (2% bovine serum albumin and 0.01% sodium azide).

Techniques: Immunohistochemistry, Expressing

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Alterations in PD-L1 Expression Associated with Acquisition of Resistance to ALK Inhibitors in ALK -Rearranged Lung Cancer

doi: 10.4143/crt.2018.486

Figure Lengend Snippet: Changes in programmed cell death–ligand 1 (PD-L1) before and after crizotinib treatment in anaplastic lymphoma kinase–positive non-small cell lung cancer tumor tissues based on H-score (A), PD-L1 score (0, 1, 2, 3) (B), and positivity (C).

Article Snippet: Cells were resuspended in 100 μL FACS buffer and then stained with mouse anti-human PD-L1 (MIH1, BD Pharmingen, San Jose, CA) or a mouse IgG1 isotype control for 30 minutes on ice in staining buffer (2% bovine serum albumin and 0.01% sodium azide).

Techniques:

Journal: Cell

Article Title: Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency

doi: 10.1016/j.cell.2017.02.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Small molecules and cytokines (purchased from Peprotech, Tocris or Santa Cruz) were supplemented as indicated at the following final concentrations: hLIF: 10 ng/ml; CHIR 99021: 1 μM; (S)-(+)-Dimethindene maleate (DiM): 2 μM; Minocycline hydrochloride (MiH): 2 μM.

Techniques: Recombinant, Knock-Out, Modification, Embryo Culture, Purification, Reporter Assay, SYBR Green Assay, Lysis, Software, High Content Screening

Journal: Journal of Inflammation Research

Article Title: Tumor-Infiltrating PD-L1+ Neutrophils Induced by GM-CSF Suppress T Cell Function in Laryngeal Squamous Cell Carcinoma and Predict Unfavorable Prognosis

doi: 10.2147/JIR.S347777

Figure Lengend Snippet: Immune cell infiltration profiles in LSCC. ( A ) Representative polychromatic dot plots displayed CD45+ leukocytes, CD3+ T cells, CD4+ T cells, CD8+ T cells γδT cells (γδTCR+) and neutrophils (CD66b+) in peripheral blood, tumor tissues and normal tissues from LSCC patients or healthy controls. ( B ) Calculative distribution of lymphocytes, neutrophils, NLR, CD3+T cells, CD4+T cells, CD8+T cells, γδT cells, and CD4+T cells /CD8 + T cells ratio in peripheral blood, tumor tissues and normal tissues. ( C ) Representative PD-1 expression on CD4+T/CD8+T/γδT cells and PDL-1 expression on CD66b+ neutrophils from the peripheral blood, tumor tissues and normal tissues. ( D ) Statistics analysis of PD-1+ CD4+T, PD-1+ CD8+T, PD-1+ γδT cells and PDL-1+ CD66b+ neutrophils. ( E ) Association analysis of TANs, NLR, CD3+ T cells or CD8+ T cells and tumor staging. ( F ) Linear correlation between TANs and CD3+ T cells, CD4+T cells, CD8+ T cells, or γδT cells. ( G ) Linear correlation between PD-L1+ TANs and PD-1+ CD4+ T cells, PD-1+ CD8+ T cells, or PD-1+ γδT cells. Each dot represents an independent data point as determined by flow cytometry. ns = p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: For flow cytometry, cell suspensions from human peripheral blood and tissue samples were stained with following antibodies cocktail at 4°C for 40 mins: Percp-Cy TM 5.5 mouse anti-human CD45 (564,105, Clone HI30, BD Pharmingen TM , San Diego, USA), BV510 mouse anti-human CD3 (564,713, Clone HIT3α, BD Pharmingen TM , San Diego, USA), APC-R700 mouse anti-human CD4 (564,975, Clone RPA-T4, BD Pharmingen TM , San Diego, USA), APC-Cy TM 7 mouse anti-human CD8 (557,834, Clone SK1, BD Pharmingen TM , San Diego, USA), PE mouse anti-human TCR γδ (555,717, Clone B1, BD Pharmingen TM , San Diego, USA), Alexa Fluor ® 647 mouse anti-human CD66b (561,645, Clone G10F5, BD Pharmingen TM , San Diego, USA), BV421 mouse anti-human PD-1 (564,323, Clone MIH4, BD Horizon TM , San Diego, USA), PE-Cy TM 7 mouse anti-human CD274 (558,017, Clone MIH1, BD Pharmingen TM , San Diego, USA).

Techniques: Expressing, Flow Cytometry

Journal: Acta Pharmaceutica Sinica. B

Article Title: A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein

doi: 10.1016/j.apsb.2020.02.009

Figure Lengend Snippet: Possible role of NF- κ B and NF- κ B-mediated pro-survival signals in TP/LPS-induced hepatotoxicity. (A) Experimental design to detect the time-dependent changes of NF- κ B and its related pro-survival factors. (B)–(G) Relative mRNA levels of NF- κ B target genes, including Ciap1 , Ciap2 , Flip L , Xiap , Tnfaip3 , and Nfkbia , were detected by qPCR with tubulin as the internal control ( n = 6). (H)–(L) Representative Western blots and relative intensity of protein bands of FLIP L , CIAP1, XIAP, and I κ B- α after the treatment of TP and LPS with tubulin as the loading control ( n = 4–6). (M) Representative photomicrographs of liver sections by IHC for P65 1 h after LPS application (200 × ). Scale bar = 50 μm. Results were expressed as mean ± SEM and statistical analysis was performed using Two-way ANOVA following by Tukey's multiple comparison test. *, # , $ P <0.05, **, ## , $$ P <0.01, ***, ### , $$$ P <0.001; ns, no statistical difference.

Article Snippet: Antibody against RIPK1 (17519-1-AP), mixed lineage kinase domain like pseudokinase (MLKL, 66675-1-Ig), myeloperoxidase (MPO, 22225-1-AP), and CIAP1 (10022-1-AP) were purchased from Proteintech (Chicago, IL, USA).

Techniques: Control, Western Blot, Comparison

Journal: Acta Pharmaceutica Sinica. B

Article Title: A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein

doi: 10.1016/j.apsb.2020.02.009

Figure Lengend Snippet: The primer sequences used for qPCR assay in mice.

Article Snippet: Antibody against RIPK1 (17519-1-AP), mixed lineage kinase domain like pseudokinase (MLKL, 66675-1-Ig), myeloperoxidase (MPO, 22225-1-AP), and CIAP1 (10022-1-AP) were purchased from Proteintech (Chicago, IL, USA).

Techniques: